Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)
Get tips on using APC anti-mouse CD274 (B7-H1, PD-L1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD274/PD-L1
Get tips on using FITC Annexin V Apoptosis Detection Kit I (RUO) to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)
Get tips on using APC Rat Anti-Mouse Ly-6G and Ly-6C to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G
Get tips on using Atg5 Antibody to perform Autophagy assay cell type - SP53
Get tips on using Atg3 Antibody to perform Autophagy assay cell type - THP 1
Get tips on using Atg3 Antibody to perform Autophagy assay cell type - SH-SY5Y
Get tips on using Atg7 Antibody to perform Autophagy assay cell type - SH-SY5Y
Get tips on using Atg3 Antibody to perform Autophagy assay cell type - KG1 cells
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
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