siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using cDNA SynthePrimeScript™ 1st strand ssynthesis Kit to perform cDNA synthesis Cell lines

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse liver organoids

Get tips on using GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker to perform DNA Ladder 100 bp

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.

Get tips on using LifeGuard Soil Preservation (1000 ml) to perform RNA stabilization Microbial

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse liver tissue

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse liver tissue

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - rat liver tissue