RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi
Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis
Get tips on using GeneRead DNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples
Get tips on using GenElute™ Plasmid Miniprep Kit to perform Plasmid Isolation S. cerevisiae
Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform
Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - C2C12 myogenin
Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Tissue - Rat Ovaries
Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - Neuroblastoma 2a Epac1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment