siRNA / miRNA gene silencing Human MDA-MB-453

Get tips on using Hs_HDAC5_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - U937 HDAC5

Get tips on using CD151 siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 CD151

Get tips on using FAK siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 FAK

Get tips on using ON-TARGETplus Human EGR1 (1958) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HCT15 Egr-1

Get tips on using SignalSilence® PTEN siRNA to perform siRNA / miRNA gene silencing Human - A2780 PTEN

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.