Protein Expression Eukaryotic cells P. pastoris

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - mouse hypothalamus tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - mouse uterus tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - mouse ovary tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - mouse oviduct tissue

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Tissue - lung

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Tissue - eye

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using RNeasy PowerSoil Total RNA Kit (25) to perform RNA isolation / purification Soil

Get tips on using MagAttract PowerSoil DNA KF Kit to perform DNA isolation / purification Tissue - fecal sample

Get tips on using AllPrep PowerViral DNA/RNA Kit (50) to perform DNA isolation / purification Viral