DNA isolation / purification Cells

Get tips on using QIAamp DNA Blood Mini QIAcube Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain

Get tips on using MagAttract 96 DNA Plant Core Kit (24) to perform DNA isolation / purification Plants - Leaves

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using mericon DNA Bacteria Kit (100) to perform DNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.