DNA methylation profiling Gene specific profiling Human ovarian tissue

Get tips on using PAXgene Tissue RNA Kit to perform RNA isolation / purification Tissue - Human Colon

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Bone DNA Purification Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Micro Kit to perform DNA isolation / purification Tissue - adipose

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using FlexiGene DNA Kit (250) to perform DNA isolation / purification Tissue - FFPE samples