rna-isolation-purification-cells-immortalized-cos-7

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RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat Experiment

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

RNA RNA isolation / purification Tissue Rat Blood / Serum / Plasma / Buffy coat

DNA isolation / purification Cells - Primary cells CD4+ T cells Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells CD4+ T cells

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A-1207