ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA-

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - THP 1

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - SH-SY5Y

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus anthracis

Get tips on using CD20 V450 to perform Flow cytometry Anti-bodies Human - CD20

Get tips on using LB Broth to perform Bacterial cell culture media Bacillus anthracis

Get tips on using pKS2-ST::CBHII to perform Protein Expression Eukaryotic cells - W. anomalus CBHII

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?