ChIP H3K27me3 Horse Rabbit

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - NRK-52E

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - MG-63

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - CaCo-2

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - C57BL/6

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.