ChIP H3K36Me3 Canine Rabbit

Get tips on using mTOR (7C10) Rabbit mAb #2983 to perform Western blotting mTOR

Get tips on using Ras (D2C1) Rabbit mAb #8955 to perform Western blotting Ras

Get tips on using TIMP2 (D18B7) Rabbit mAb #5738 to perform Western blotting TIMP-2

Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Get tips on using Beclin 1 Rabbit Polyclonal antibody to perform Autophagy assay cell type - A375

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - A2780

Get tips on using SQSTM1/p62 (D1Q5S) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.