ChIP acH4 Donkey Rabbit

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-53BP1 (phospho S25) antibody, rabbit polyclonal to perform Immunohistochemistry 53BP2 phospho (ser-25) - Rabbit IgG Human -NA-

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HK-2 cells

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.