ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA- Human

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - FaDu

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HaCaT

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Get tips on using MouseTRAP™ (TRAcP 5b) ELISA to perform Acid phosphatase assay cell type - murine macrophage cells

Get tips on using β-Gal Assay Kit to perform Reporter gene assay β-galactosidase substrates - PANC-1

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.