Select a Cell type


DNA isolation / purification Bacteria

- Found 5258 results

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

DNA Plasmid Isolation Salmonella enterica serovar Indiana (S. Indiana)

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Products Qiagen QIAamp DNA Mini Kit

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

Products Qiagen QIAamp DNA Mini Kit

RNA RNA isolation / purification Tissue Human Colon

RNA RNA isolation / purification Tissue Mouse Vagina

RNA RNA isolation / purification Tissue Human Saliva

RNA RNA isolation / purification Viral Dengue virus

RNA RNA isolation / purification Viral Hazara virus

RNA mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Primary cells Lymphocytes

Products Qiagen QIAamp DNA Blood Mini Kit

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms