DNA methylation profiling Gene specific profiling HEK 293T

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - HEK 293

Get tips on using Supermix DNA Ladder to perform DNA Ladder 500 bp

Get tips on using 100bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using YeaStar™ Genomic DNA Kit to perform DNA isolation / purification Yeast - Pichia pastoris

Get tips on using YeaStar™ Genomic DNA Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using Chromous Genomic DNA isolation kit to perform DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Get tips on using DreamTaq DNA Polymerases to perform PCR Conventional / Qualitative PCR - bacterial DNA