siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using SignalSilence® IκBα siRNA to perform siRNA / miRNA gene silencing Human - HT-29 IκBα

Get tips on using SignalSilence® FoxO3a siRNA to perform siRNA / miRNA gene silencing Human - Calu-3 FOXO3

Get tips on using SignalSilence® FoxO1 siRNA to perform siRNA / miRNA gene silencing Human - Calu-3 FOXO1

Get tips on using SignalSilence® IκBα siRNA to perform siRNA / miRNA gene silencing Human - Caco-2 IκBα

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Rab Coupling Protein (RCP)

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Stealth siRNA(h)_GATA1 to perform siRNA / miRNA gene silencing Human - LAD2 GATA1

Get tips on using Silencer® Select_SPRY2 siRNA to perform siRNA / miRNA gene silencing Human - RMS SPRY2

Get tips on using CREB-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC CREB