3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

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Found 3 matching solutions for this experiment

Upstream tips
You can develop chemoresistant cell lines
Protocol tips
Seed 1.0 ×10 4 cells/well
Use of 24-ultralow attachment plates
Analysis of spheres with a diameter >40 μm
Downstream tips
Collect spheres for other procedures, such as RNA isolation
DMEM/F-12, no phenol red

Thermo Fisher Scientific

Protocol tips
Seed 1x10 3 cells/well
MammoCult™ Human Medium Kit

STEMCELL technologies

Protocol tips
Seed at a density of 5,000 cells/mL
Use 6-well ultra-low-attachment plates
Count mammospheres ≥50 μm in diameter
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