siRNA / miRNA gene silencing Human MCF-7

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Arp2 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D Arp-2

Get tips on using siRNA TICAM-1 to perform siRNA / miRNA gene silencing Human - HT-29 TICAM-1

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using Gβ 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D GNB1

Get tips on using Stealth siRNA™ NOTCH2 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH2

Get tips on using Stealth siRNA™ NOTCH1 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH1

Get tips on using caveolin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B CAV1

Get tips on using Flotillin-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B FLOT2

Get tips on using Rab 5C siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB5C