DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using STEMdiff™ APEL™ 2 Medium to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using STEMdiff™ APEL™ 2-LI Medium to perform Stem cell Differentiation media hESCs or hiPSCs differentiation into Cardiomyocytes

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using FastDigest BsuRI to perform Restriction Enzymes HaeIII / BsuRI

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Haemophilus parasuis

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2