Protein isolation

Get tips on using CelLytic™ B Plus Kit to perform Protein isolation Bacteria - Escherichia coli

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Anabaena

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Synechocystis

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - Rat_Mesenteric fat