Immunohistochemistry Collagen VII [II-32] Mouse

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Get tips on using Anti-SOX9 antibody produced in rabbit to perform Immunohistochemistry Human - SOX9

Products Sigma-Aldrich Anti-SOX9 antibody produced in rabbit

Get tips on using Anti-DICER1 antibody produced in rabbit to perform Immunohistochemistry Human - Dicer1

Products Sigma-Aldrich Anti-DICER1 antibody produced in rabbit

Get tips on using PR Antibody (F-4): sc-166169 to perform Immunohistochemistry Human - PR

Products Santa Cruz Biotechnology PR Antibody (F-4): sc-166169

Get tips on using Estrogen Receptor alpha Monoclonal Antibody (SP1) to perform Immunohistochemistry Human - ER

Products Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (SP1)

Get tips on using CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody to perform Immunohistochemistry Human - CDX2

Products Cell Marque Tissue Diagnostics CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Immunohistochemistry Rat - Sox9

Products Abcam Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

Products Cell Signaling Technology MMP-3 (D7F5B) Rabbit mAb #14351

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

Products Abcam Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451)

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Mouse - HSP70

Products R&D Systems Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells mouse splenocytes

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