Protein quantification Mammalian cells

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD8+ T cells

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD4+ T cells

Get tips on using Qproteome Nuclear Protein Kit to perform Protein enrichment Soluble nuclear proteins

Get tips on using Dihydroethidium to perform DNA quantification Human - SH-SY5Y

Get tips on using Hoechst 33342 to perform DNA quantification Human - HeLa

Get tips on using QuantiTect Virus Kit to perform RNA quantification qPCR

Get tips on using Unstained Protein Standards to perform Protein Ladder Unstained

Get tips on using Prestained Protein Standards to perform Protein Ladder Prestained

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Cell Counting Kit-8 to perform RNA quantification Coloremetric