dna-isolation-purification-yeast-candida-albicans

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QIAGEN Plasmid Plus Midi Kit (25) Product

Get tips on using QIAGEN Plasmid Plus Midi Kit (25) to perform DNA isolation / purification Plasmid purification

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Protein isolation Tissue - Mouse cardiac tissue Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Tissue Mouse cardiac tissue

DNeasy PowerWater Kit (100) Product

Get tips on using DNeasy PowerWater Kit (100) to perform DNA isolation / purification Water samples

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DNeasy PowerBiofilm Kit (50) Product

Get tips on using DNeasy PowerBiofilm Kit (50) to perform DNA isolation / purification Biofilm samples

Products Qiagen DNeasy PowerBiofilm Kit (50)

DNeasy Blood & Tissue Kit Product

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose