rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

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siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP2B6 Experiment

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

RNA siRNA / miRNA gene silencing Human Primary Human Hepatocytes CYP2B6
Endothelial Cell Medium Product

Get tips on using Endothelial Cell Medium to perform Mammalian cell culture media CADMEC/HMVEC

Products ScienCell Research Laboratories Endothelial Cell Medium
miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

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miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

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SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MCF-7

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Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized U-251

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SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Saos-2

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High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

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High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized K-562

Products Roche Lifesciences High Pure RNA Isolation Kit
SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized HT-29

Products Promega SV Total RNA Isolation System

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