CRISPR Rat Deletion INS-1 832/13

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using BIO-MPM-1 SFM to perform Mammalian cell culture media HC

Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549

Get tips on using TriDye™ 1 kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Mucin 1 Antibody (VU4H5): sc-7313 to perform Immunohistochemistry Human - Muc-1

Get tips on using caveolin-1 Antibody (7C8): sc-53564 to perform Western blotting Caveolin-1

Get tips on using claudin-1 Antibody (XX7): sc-81796 to perform Western blotting Cclaudin-1

Get tips on using Mouse Dkk-1 Quantikine ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Human HO 1 ELISA Kit (ab133064) to perform ELISA Human - HO-1