ROS assay cell type - rat kidney and pancreas tissue

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Upstream tips
- Dilute compounds of interest in complete media without phenol red.
Protocol tips
- Incubate DCFDA and compounds for the desired period of time (30 – 45 minutes).

- 1 hour prior to completion of the treatment, dilute DCFDA at 2X of the final concentration desired in the same media used for treatment
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