Protein Expression Eukaryotic cells N. benthamiana

Get tips on using Bicinchoninic Acid Kit for Protein Determination to perform Protein quantification Mammalian cells - 3T3

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - 3T3

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - C2C12

Get tips on using Bicinchoninic Acid Kit for Protein Determination to perform Protein quantification Mammalian cells - C2C12

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HeLa

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - SiHa

Get tips on using Total Exosome RNA & Protein Isolation Kit to perform Protein isolation Mammalian cells - HeLa

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.