Protein Expression Prokaryotic cells L. citreum

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Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

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DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays A-375 human melanoma Digoxigenin-11-dUTP

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Rat pancreas tissue Cyanine 3 & cyanine 5

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Proteins Protein Ladder IEF and 2-D Standards

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - PC-12

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Rat microglia

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human chondrocytes

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - BV-2

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - BV-2

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - J774A.1

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

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