Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - PANC-1 PKN3

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - PANC-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - PANC-1 hENT1

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - Capan-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - Capan-1 hENT1

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-1

Get tips on using EZ-ChIP™ to perform ChIP Human - PANC-1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.