Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Subcutaneous
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Stomach
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Spleen
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Retina
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Liver
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Intestine
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Gut
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
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