shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using Stealth siRNA(m) Atg16l2 to perform siRNA / miRNA gene silencing Mouse - Pancreatic Acinar cells Atg16l2

Get tips on using SMARTpool: siGENOME Fancd2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16 Melanoma cells FANCD2

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Blood & Cell Culture DNA Midi Kit (25) to perform DNA isolation / purification Cells - Immortalized cell lines Human Neuroblastoma Cell Lines

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - DU145

Get tips on using Stealth siRNA(m)_Atg5 to perform siRNA / miRNA gene silencing Mouse - MLO‐Y4 Atg5

Get tips on using IRF-1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 IRF1

Get tips on using HIF-1α siRNA (m) to perform siRNA / miRNA gene silencing Mouse - AtT20 Hif-1alpha