siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using LDH-Cytotoxicity Colorimetric Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y

Get tips on using Cytotoxicity LDH Assay Kit-WST to perform Cell cytotoxicity / Proliferation assay cell type - L-02

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - FTC133

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - FTC236

Get tips on using 3 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - Jurkat

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Cell migration / Invasion cell type - HaCat

Get tips on using 5 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - A549

Get tips on using Pierce™ LDH Cytotoxicity Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - HEK 293

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.