ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA-

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 Mt-PA

Get tips on using ExpiCHO™ Expression System Kit to perform Protein expression and purification Mammalian cells - CHO-K1 SUMO protein

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus paracasei

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus helveticus

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus plantarum

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat ileum

Get tips on using DMEM:F12 with HEPES, Glucose and L-Glutamine to perform 3D Cell Culture Media Human lung cancer organoids

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 ROCK2

Get tips on using pFastBac1-sccaIL12opt to perform Protein Expression Eukaryotic cells - Hi5 IL12 canine