Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using TACS® 2 TdT Fluorescein Kit to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using Acridine Orange - CAS 65-61-2 - Calbiochem to perform Necrosis HUVEC

Get tips on using Minimum Essential Medium Eagle to perform Stem cell culture media Rat BMSC

Get tips on using Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508) to perform Immunohistochemistry Mouse - Lysozyme

Get tips on using PARP-1 Antibody (F-2): sc-8007 to perform Western blotting PARP

Get tips on using Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411) to perform Western blotting HDAC1

Get tips on using Anti-Mucin 5AC antibody [2-11M1] (ab24071) to perform Western blotting MUC5AC

Get tips on using beta Tubulin Monoclonal Antibody (2 28 33) to perform Western blotting Tubulin beta

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Stealth siRNA(r)_Plat to perform siRNA / miRNA gene silencing Rat - NPC tPA/Plat