Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - rat kidney tissue
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Rat Gut
Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis
Get tips on using High Pure miRNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bone marrow
Get tips on using Maxwell® 16 LEV simplyRNA Purification Kit to perform RNA isolation / purification Tissue - Human Muscles
Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue
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