siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using ON-TARGETplus Human MET (4233) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 MET

Get tips on using ON-TARGETplus Human LYVE1 (10894) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 LYVE-1

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using ON-TARGETplus Human NCR3LG1 (374383) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HT-29 B7-H6/NCR3LG1

Get tips on using ON-TARGETplus Human SLC7A5 (8140) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MDA-MB-231 LAT1/SLC7A5

Get tips on using ON-TARGETplus Human PLK1 (5347) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 PLK-1

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - H1299 Rab Coupling Protein (RCP)

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 Rab Coupling Protein (RCP)

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.