siRNA / miRNA gene silencing Human Primary Human Hepatocytes

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - Capan-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - Capan-1 hENT1

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - SW1990 DNMT1/3b

Get tips on using STEAP2 metalloreductase to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) STEAP2

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 PKN3

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.