siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using RNeasy PowerClean Pro Cleanup Kit (50) to perform Removal of contamination in RNA Melanin contamination

Get tips on using QIAamp MinElute Virus Vacuum Kit (50) to perform RNA isolation / purification Viral - Viral CNS disease

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR - fibroblast

Get tips on using Quick Amp Labeling Kit-one color to perform Microarray RNA amplification & Labeling - Mouse cochlaea Cyanine CTP

Get tips on using RiboMinus™ Transcriptome Isolation Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using QIAamp 96 Virus QIAcube HT Kit (5) to perform RNA isolation / purification Viral - SARS-CoV-2

Get tips on using Quick Amp Labeling Kit-one color to perform Microarray RNA amplification & Labeling - Mouse Myofibers Cy3- or/and Cy5