dna-isolation-purification-bacteria-gram-positive-clostridium-difficile

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using GenElute™ Bacterial Genomic DNA Kit to perform DNA isolation / purification Bacteria - Gram positive Actinomycytes

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis