RNA isolation / purification Tissue Human

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Mouse Adipose

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat brain tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat spleen tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat lung tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat liver tissue

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - rat pancreas tissue

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Rat Pineal gland

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.