Protein Expression Prokaryotic cells M. smegmatis

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat lung tissue

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat liver tissue

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat pancreas tissue

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - MDA-MB-231

Get tips on using Quick Amp Labeling Kit-one color to perform RNA amplification & labeling Mammalian - RNA, Mice Cochlaea Cyanine CTP

Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Quick-Load® Purple Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range

Get tips on using miRNeasy Serum/Plasma Advanced Kit (50) to perform RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat