siRNA / miRNA gene silencing Human siRNA negative control

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Get tips on using Atf4 siRNA(m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4

Get tips on using Rn_Rab7_5 FlexiTube siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Rab7

Get tips on using siRNA(r) NM_012820 Acsl1 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl1

Get tips on using siRNA(r) NM_057107 Acsl3 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl3

Get tips on using siRNA(r) NM_053623 Acsl4 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl4

Get tips on using Stealth siRNA(m) Stac3 to perform siRNA / miRNA gene silencing Mouse - C2C12 Stac3

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Pericytes Cx43

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Stealth siRNA(r)_Plat to perform siRNA / miRNA gene silencing Rat - NPC tPA/Plat