As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Acridine Orange - CAS 65-61-2 - Calbiochem to perform Necrosis HUVEC
Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis HeLa
Get tips on using TACS Annexin V-FITC Apoptosis Detection Kit to perform Necrosis MCF7
Get tips on using Annexin V-FITC Kit, 200 Tests, RUO to perform Necrosis MCF7
Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis MCF7
Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis A549
Get tips on using Candida albicans antigen ELISA Kit to perform Cell Culture Contamination Detection Kit Yeast
Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Yeast
Get tips on using Human Candida Albicans ELISA Kit to perform Cell Culture Contamination Detection Kit Yeast
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment