siRNA / miRNA gene silencing Human CRL-5915

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using FastDigest Eco31I (IIs class) to perform Restriction Enzymes BsaI / Eco31I

Get tips on using Anti-NSE, Clone MIG-N3 to perform Immunohistochemistry Mouse - NSE

Get tips on using Anti-CD133 Antibody, clone 13A4 to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-Somatostatin Antibody, clone YC7 to perform Immunohistochemistry Mouse - Somatostatin

Get tips on using Anti-Glucagon Antibody, clone 13D11.33 to perform Immunohistochemistry Mouse - Glucagon

Get tips on using Anti-Fas Antibody, clone 7C10 to perform Western blotting Fas

Get tips on using Anti-NOD2 Antibody, clone 2D9 to perform Western blotting NOD2

Get tips on using Anti-podoplanin, Clone RTD4E10 to perform Immunohistochemistry Podoplanin - Hamster Mouse -NA-