When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes
Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes
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