Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris

Get tips on using Anti-Integrin β1 Antibody, clone MB1.2 to perform Flow cytometry Anti-bodies Mouse - CD29/β1-Integrin

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion MDA-MB-231 sodium channel β1 subunit

Get tips on using Investigator Quantiplex HYres Kit to perform DNA quantification Blood

Get tips on using PyroMark CpG Assays to perform DNA methylation profiling Whole genome profiling - human whole blood

Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Mammalian cell culture media MA-104

Get tips on using QIAamp MinElute ccfDNA Mini Kit (50) to perform DNA isolation / purification Tissue - blood / plasma

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using PowerPlex® 18D System to perform Cell line authentication Human iPSC cells derived from peripheral blood mononuclear cells