siRNA / RNAi /miRNA transfection Human Cells Primary splenocytes

Get tips on using ON-TARGETplus Human NBN (4683) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MCF-7 NBS1/NBN

Get tips on using ON-TARGETplus Human RAD51 (5888) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 RAD51

Get tips on using ON-TARGETplus Human AHR (196) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MDA-MB-231 AHR

Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 PACE4

Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 Furin

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 PC7

Get tips on using ON-TARGETplus Human MET (4233) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 MET

Get tips on using ON-TARGETplus Human LYVE1 (10894) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 LYVE-1

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.