Found 1 discussion for this experiment
2 years ago
2 years ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 3 matching solutions for this experiment
|The following might help to increase RNA yield
- Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes.
- Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration).
- Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).