Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres
Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres
Get tips on using IMAGEN™ Herpes Simplex Virus (HSV) Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus
Get tips on using IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus
Get tips on using EasySep™ Human Naïve B Cell Enrichment Kit to perform Cell Isolation Naive B cell
Get tips on using MojoSort™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell
Get tips on using EasySep™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell
Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres
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