rna-isolation-purification-tissue-mouse-spine

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat brain tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat spleen tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using RNeasy DSP FFPE Kit to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse prostate tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse lung tissue

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.